Quantitative determination of the content of venlafaxine and its active metabolite in human plasma using liquid chromatography-mass spectrometry

A validated method for the quantitative determination of venlafaxine, O-desmethylvenlafaxine in human plasma by tandem liquid chromatographymass spectrometry (HPLC/MS/MS) has been proposed. Sample preparation was carried out liquid-phase extraction with support (SLE, from «supported extraction») using HyperSEP cartridges and washing 3-methylbutyl ether. The measure target analytes on a triple quadrupole mass spectrometer under multiple-reaction monitoring (+MRM) mode electrospray ionization. calibration curves were linear regression coefficient r 2 > 0.999. validation showed high sensitivity, specificity, accuracy reproducibility, as well stability after freeze/thaw cycles storage at –20 °C. is developed therapeutic drug monitoring.